HaloTag

Compounds and compositions comprising fluorophores for use in visualization and purification or manipulation

The application discloses a compound having a moiety that is either an affinity tag-containing moiety or a protein-manipulation moiety, …

Luke D. Lavis, Pratik Kumar, Carla Nicolini, Jonathan B. Grimm

Last updated on Feb 17, 2025

Optimizing multifunctional fluorescent ligands for intracellular labeling

Enzyme-based self-labeling tags enable covalent attachment of synthetic molecules to proteins inside living cells. A frontier of this …

Pratik Kumar, Jason D. Vevea, Ariana N. Tkachuk, Kirby Campbell, Emma T. Watson, Anthony X. Ayala, Jonathan B. Grimm, Edwin R. Chapman, David J. Solecki, Luke D. Lavis

Biotin-free proximity labeling

Compounds useful for biotin-free proximity labeling are provided. Also provided are methods for biotin-free labeling of biomolecules in …

Shu-Hsien Sheu, Pratik Kumar, Luke D. Lavis

Last updated on Feb 17, 2025

Single-cell synaptome mapping of endogenous protein subpopulations in mammalian brain

Motokazu Uchigashima, Risa Iguchi, Kazuma Fujii, Kaito Shiku, Pratik Kumar, Xinyi Liu, Mari Isogai, Chiaki Hoshino1, Manabu Abe, Motohiro Nozumi, Yosuke Okamura, Michihiro Igarashi, Kenji Sakimura, Ryoma Bise, Luke Lavis, Takayasu Mikuni